Some fibroblasts have a contractile function; these are called myofibroblasts. They phagocytose cell debris and/or foreign material. Loose (areolar connective tissue) is the most abundant form of collagenous connective tissue. Ground substance is an aqueous gel of glycoproteins and proteoglycans that occupies the space between cellular and fibrillar elements of the connective tissue. These are fibroblasts View Image (as opposed to clusters of similar appearing elongate nuclei that are usually smooth muscle cells or Schwann cells in a nerve that you will learn to recognize soon). Look at the areas outlined in the orientation diagram of the trachea and locate the loose, cellular connective tissue within the glands (the "glands" are coiled tubes of columnar epithelial cells; some the epithelial cells are tall and eosinophilic, whereas others are shorter and more basophilic). You should note that the cytoplasm is quite basophilic (i.e. The collagen fibers (cf) are parallel to the arrow bar. If you are a University of Michigan student enrolled in a histology course at the University of Michigan, please click on the following link and use your Kerberos-password for access to download lecture handouts and the other resources. The background will be clear, reflecting a "ground substance"-rich connective tissue. Tissue macrophages can be found in many different organs. Be able to recognize interstitial (fibrillar) collagens and elastic fibers at the light and electron microscopic levels. You should be able to recognize a range of nuclear morphologies and be able to identify the cells as fibroblasts. Stratified squamous non-keratinizing epithelium lines the pharynx. Now with slide 28 (make sure your slide is stained with silver; it should say “Ag” on the label! See more ideas about tissue, things under a microscope, fun facts. Protein secreting cells have a basophilic cytoplasm because they are full of rough ER, which stains with hematoxylin, a basic dye. This is loose connective tissue. These tissues have two principal components, an extracellular matrix and a variety of support cells. White fat cells or adipocytes are specialized for the storage of triglyceride, and occur singly or in small groups scattered throughout the loose connective tissue. Stratified squamous keratinized epithelium, Stratified squamous non-keratinized epithelium. Many of the free cells in these medullary sinuses cannot be identified; however, the large rounded cells, with eccentrically placed, vesicular nuclei are the ones you should try to find. Get started! Loose (areolar connective tissue) is the most abundant form of collagenous connective tissue. In contrast to epithelia, connective tissue is sparsely populated by cells and contains an extensive extracellular matrix consisting of protein fibers, glycoproteins, and proteoglycans. Overview of Connective Tissue. The fine collagenous network that provides support in the bone marrow, lymphatic organs, around individual smooth muscle cells, and beneath most epithelia is composed mainly of Type III collagen. Fluid connective tissue. Note glass appearance and relatively fewer cells than in elastic cartilage. Then use your text and atlas to review the diagnostic features of each connective tissue cell present in the micrograph. Fibroblasts are large, flat, elongated (spindle-shaped) cells possessing processes extending out from the ends of the cell body. Mast cells are most abundant in the connective tissue associated with the lining of the digestive and respiratory systems, and your collection just so happens to contain a tissue section from the stomach that has been stained with PAS and Azure II. Elastic cartilage is characterized by the presence of abundant elastic fibers and is quite cellular. It is made up of type II collagen and is located in the auricle of the ear and the epiglottis. It provides metabolic support (blood vessels), immune support (white blood cells) and structural support. Stain results: Septa: Red: ... Electron Microscopy Sciences specializes in the manufacturing, preparation and distribution of the highest quality laboratory chemicals and microscopy supplies and equipment for electron microscopy, light microscopy, and histology. Three kinds of cartilage are classified according to the abundance of certain fibers and the characteristics of their matrix. Start studying Lab 4: Connective Tissue Microscope Slides. Large amounts of rough ER indicate that the cell is active and is producing large amounts of proteins. At high magnification, the larger strands are seen to be made up of bundles of smaller fibres. Histology of Connective Tissues View Online Download for PC for Mobile Contents General Characteristics Structure Functions Matrix Dense connective … Neutrophils generally enter tissues in large numbers only in response to a disease stimulus. These are reticular fibers, found in skin, muscle and blood vessels. They have numerous, smaller lipid droplets and a large number of mitochondria, whose cytochromes impart the brown color of the tissue. The very small cracks between the fibers are just artifacts of shrinkage that occured during tissue preparation. Unlike the tissue of the aorta which would be classified as "regular," these tissues are obviously irregular but they're mentioned here for the purpose of illustrating how aggregates of elastic fibers appear in H&E-stained sections. Some of the individual fat cells are often broken during tissue preparation, but the overall impression of what the tissue looks like is the important point. You are not responsible for recognizing reticular fibers unless a silver stain is used. These cells are abundant in newborns and hibernating mammals, but are rare in adults. Mast cells contain a mixture of granule types reflective of the variety of substances they secrete. They phagocytose cell debris and/or foreign material. Dense regular connective tissue 400X Tendon In this image a fibroblast nucleus (fb nuc) is labeled, but you can see other nuclei once you know what to look for. Note the coarse chromatin clumps organzed as radial spokes in the round nuclei which is therefore often described as having a "clockface" or "wagon-wheel" appearance. Make sure you can see the difference between cross sectioned and longitudinally sectioned collagenous fibrils. Heparin and histamine are just some of the contents of mast cell granules. 7 Types of Connective Tissue - Microscope Slides learn by taking a quiz; Online quiz to learn 7 Types of Connective Tissue - Microscope Slides; Your Skills & Rank. They both have all three of the defining characteristics listed above, but their extracellular matrix is tougher, denser, and more solid than the various types of connective tissue proper. Elastic fibers contain the protein elastin, which co-polymerizes with the protein fibrillin. Chapter 3 - Connective Tissue. The function of this type of tissue is to provide structural and mechanical support for other tissues, and to mediate the exchange of nutrients and waste between the circulation … Use these micrographs to review the structure of organelles. Mast cells are actively involved in a host's immune response and produce many substances, some of which are heparin and histamine. The reticular fibers provide physical support for all the cells present in tissues subject to stretching. Total Points. In this area note the irregular, wavy collagen fibers arranged singly or in small groups. The extracellular substance consists of fibres which are embedded in ground substance containing tissue fluid. (CT9) Test your ability to identify different organelles at this magnification! These streaks (arrows) are usually thin cytoplasmic extensions of fibroblasts, whose cell bodies are not in the plane of section. Thus, what distinguishes the different connective tissues is the type of matrix. Fibroblasts are by far the most common native cell type of connective tissue. Aug 30, 2014 - This Pin was discovered by Haley Boyer. Blood and lymph are the two types of connective tissue in this sub-category. 2. Macrophages can be seen also in the subcapsular sinus (the lighter staining area just under the capsule at the periphery of the lymph node). The basal lamina is anchored to the underlying connective tissue by fine fibrils oftype VII collagen (you obviously can't tell this looking at it in the light microscope, but you should recall this from lecture). Epithelial Tissue: Epithelial tissue is found in the skin, glands, organs, mucous membranes, and organs like kidney and lungs. When the IgE binds an appropriate molecule, it will trigger degranulation of the mast cell and the vasodilation, congestion, bronchiolar constriction, wheezing, etc. Cartilage is a specialized form of connective tissue produced by differentiated fibroblast-like cells called chondrocytes. They are derived from mesenchymal progenitor cells. Classify the connective tissue and describe its function. Correct answer 5. It develops from the mesoderm. Remember that each fat cell is enclosed by a thin basal lamina (Unfortunately, in these examples you can’t see the basal lamina). Dense Connective Tissue. Locate and classify the connective tissue in this image of the intestine. Connective tissue is made up of cells and extracellular matrix. Today's Rank--0. The antibody binds mast cells and waits for a second exposure to whatever it happens to be responsive to (an allergen). Hyaline Cartilage. Download this Dense Connective Tissue In Microscope vector illustration now. This region is also a loose, irregular connective tissue but can be so extensively infiltrated by white blood cells and plasma cells that the supporting fibers and ground substance are obscured. Connective tissue is found in between other tissues everywhere in the body, including the nervous system. However, to see the mast cells, you will need to look deeper in the submucosa where you should find small, ovoid cells amongst the collagen fibers with spherical, eccentric nuclei and intensely basophilic (dark purple to black) granulesView Image. Adipose tissue is an exception among connective tissues in that the cells are packed together and there is no large amount of matrix. Resists tension in one direction. What are the secretory products of the mast cell? Plasma cells may also be foundwithin the connective tissue of many of the glands that secrete into these regions. These cells are important in removing all kinds of debris from the body as well as playing a major role in the immune response. Typical specimens in pathology are colon, kidney, pancreas, cervix, lung, breast, prostate, or connective tissue. Slide 29 (small intestine, H&E) View Virtual Slide. Correct answer 4. Non-striated (involuntary) muscle E 5 Striated muscle, showing striations and nuclei LS 5 Adipose tissue, section 5 Pseudo-stratified ciliated: epithelium TS 5 Areolar tissue, stained for fibres and cells E 5 Spinal cord TS 5 Compact bone, for Haversian Canals and lamellae 5 Cardiac muscle showing the nuclei, striations and intercalated discs LS 5 This cartilage is found in the nose, tracheal rings, and where the ribs join the sternum. Now look at the region immediately underneath the intestinal epithelium, which is called the lamina propria View Image . Brown fat cells would have several small lipid droplets all of which would be roughly the same size. In the region immediately beneath the epidermis you can see how the elastic fibers are interconnected forming an elaborate, delicate net of fibers View Image amongst thin strands of collagen. Fibrocartilage is distinguished by its high content and orderly arrangement of type I collagen fibers. Be able to distinguish between type I collagen, type III (reticular) collagen, and elastic  fibers when appropriately stained material is presented. "loose" versus "dense" connective tissue), sometimes it is not always possible to classify connective tissue in a given section; it may be a little loose, a little dense, a little fatty, etc. Most frequently, the different types of connective tissues are specified by their content of three distinguishing types of extracellular fibers: collagenous fibers, elastic fibers, and reticular fibers. A fatty tissue called brown or multilocular fat, produced during fetal development, has adipocytes that contain multiple fat droplets. Learn the terms, keywords, vocabulary, and much more about Microscopic Images of Tissues with our flashcards quizzes. Game Points. Be able to describe the functions of cells commonly found in connective tissue and identify them. Unlike epithelium, connective tissues do not have a free surface. Image of blue, connective, loose - 130916821 Connective tissue is one of the four basic types of animal tissue, along with epithelial tissue, muscle tissue, and nervous tissue. Connective tissue fills the spaces between organs and tissues, and provides structural and metabolic support for other tissues and organs. Health Information Technology & Services. Unlike the thick and coarse collagenous fibers, reticular fibers form a thin reticular network. When sectioned material is observed with the light microscope, Dense Irregular Connective Tissue is often seen to contain small, thin, dark streaks that contrast with the surrounding pale-staining Collagenous Fibers. Connective tissue - blood (Note: Erythrocytes - red blood cells; have hemoglobin; don't have nuclei because they lose it when made from bone marrow. Look for more examples of fibroblasts and note that you rarely see much cytoplasm and that the nuclei of these cells can be quite condensed and hyperchromatic depending upon the plane of section or the metabolic state. The nuclei of many cells are not included in the field of view. Leukocytes - white blood cells; have nucleus; dyed purple to distinguish under microscope.) Areolar Connective Tissue The fibroblast synthesizes the collagen and ground substance of the extracellular matrix. 21 Plasma cell View Virtual EM Slide 29 Tendon - Longitudinal section View Virtual EM Slide In dense connective tissue, which type of cell is most common? Ground substance is an aqueous gel of glycoproteins and proteoglycans that occupies the space between cellular and fibrillar elements of the connective tissue. A final, special connective tissue is vascular or blood tissue. 158-197, Ross and Pawlina (6th ed), Chapter 9 Adipose Tissue, pgs. These cells mediate immune responses to foreign particles. It occurs in small, elongated bundles separated by regions that contain ground substance. Fibroblast, the principal active cell of connective tissue. Look for adipose tissue in Slide 30 which is taken from abdominal mesentery (the connective tissue that suspends the viscera within the abdominal cavity). 4. Even though we try to set up rigid categories (e.g. Of course, there are some places where there are breaks in the dense regular connective tissue of the tendon containing loose connective tissue associated with nerves and blood vessels or the occasional bit of adipose tissue. Recall that the primary function of plasma cells is antibody secretion, so they are a prominent constituent of loose connective tissue wherever antigens may enter the body, such as the gastrointestinal, urogenital, and respiratory tracts. This quiz only covers material that one would be expected to discuss in a histology laboratory. Microscope at 400X. Look for plasma cells within the lamina propria of slide 29 View Image. Slide 152 (pharynx, H&E) View Virtual Slide, Slide 30 (mesentery, H&E) View Virtual Slide, Slide H2 (fetal thorax, H&E) View Virtual Slide (virtual slide courtesy of Western University). As you look at this section, you will see a very obvious layer of mucous epithelial cells (PAS also reacts with the carbohydrate-rich mucin). (CT7), 18 Loose Connective Tissue - Lamina propria of tracheal mucosa View Virtual EM Slide When fat cells have accumulated in such abundance that they crowd out or replace cellular and fibrous elements, the accumulation is termed adipose tissue. The extracellular matrix is made up of fibres in a protein and polysaccharide matrix, secreted and organised by cells in the extracellular matrix. Observe the mixture of collagen and elastic fibers in this cross section of chorda tendinea. Correct answer 3. These are termed reticular (or rarely, argyrophilic= "silver loving") fibers. & Mass. Which statement about the cells marked by the black arrows is true? These cells can grow up to 100 microns and usually contain once centrally located vacuole of lipid - the cytoplasm forms a circular ring around this vacuole, and the nucleus is compressed and displaced to the side. Slide 26 (lymph node, H&E) View Virtual Slide. Chondrocytes are located within lacunae in the matrix that they have built around themselves. In biology, tissue is a cellular organizational level between cells and a complete organ.A tissue is an ensemble of similar cells and their extracellular matrix from the same origin that together carry out a specific function. The English word "tissue" derives from the French word "tissue", meaning that … An example of regular dense connective tissue is that of tendons; an example of irregular dense connective tissue is that of the dermis. In this image of an artery, What type of fibers are stained darkly? 26 Connective Tissue - Dense Irregular View Virtual EM Slide Discover (and save!) Location. Brown fat cells are highly specialized for temperature regulation. Histamine and heparin are found in the more "regular" looking granules (evenly dark and round). Be sure you look at both the H&E and Masson trichrome-stained slides as they provide an excellent opportunity to see how collagen stains in connective tissue when either stain is applied. 28 Collagen and Elastin - Cross section of Chorda Tendinea View Virtual EM Slide In particular, they release large amounts of histamine and enzymes in response to antigen recognition. Where are the nuclei of the fat cells? 25 Connective Tissue - Fibroblast View Virtual EM Slide Observe the branching nature of the elastic fiber and the "mantle" of elastic microfibrils. 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